lncRNACNN3-206 activates intestinal epithelial cell apoptosis and invasion by sponging miR-212, an implication for Crohn's disease

Figure 4 Results of luciferase reporter assays. A: Results of luciferase reporter assays following co-transfection of Caco-2 cells with a luciferase plasmid vector containing the 3' end of lncRNACNN3-206 and lentivirus expressing miR-212 mimics (left), or a luciferase plasmid vector containing the 3’ UTR of Caspase10 and lentivirus expressing miR-212 mimics (right). Note the significant inhibition of luciferase activity in the wild-type constructs by miR-212 mimic overexpression. PsiCHECK-2 is the name of an empty plasmid, and served as a control for both lncRNA CNN3-206 and Caspase 10; B: Illustration of the ternary mechanism for the interactions among lncRNACNN3-206, miR-212 and Caspase10. ^aP < 0.05, ^bP < 0.01.