lncRNACNN3-206 activates intestinal epithelial cell apoptosis and invasion by sponging miR-212, an implication for Crohn's disease

Figure 3 Detection of lncRNACNN3-206 by in situ hybridization. Green fluorescence was generated by a lncRNACNN3-206 probe. Red fluorescence was generated by a probe for CK20, a molecular marker of intestinal epithelial cells (yellow arrows). Blue fluorescence was from DAPI staining of nuclei (pink arrows). LncRNACNN3-206 was highly expressed in the intestinal mucosa from Crohn’s disease patients compared to normal intestinal mucosa. Further observation at high magnification revealed that lncRNACNN3-206 was mainly distributed in the cytoplasm of intestinal epithelial cells (white arrows). Magnifications are: 100 × on the left, and 400 × on the right. CD: Crohn’s disease.