Role of doublecortin-like kinase 1 and leucine-rich repeat-containing G-protein-coupled receptor 5 in patients with stage II/III colorectal cancer: Cancer progression and prognosis

Table 3 Prognostic value, univariate and multivariate analyses of potential predictor for recurrence

Variables	HR	95%CI	P value
Univariate
Age (≤ 49 vs > 49 yr)	0.742	0.303-1.818	0.515
Gender (Male vs Female)	0.758	0.302-1.901	0.555
Depth of penetration (T3 vs pT4)	1.310	0.383-4.476	0.006
Lymph node
N0	1 (ref)
N1	2.590	0.795-8.439	0.114
N2	7.562	2.189-26.115	0.001
Tumor stage (TNM) (II vs III)	3.610	1.201-10.849	0.022
Differentiation (well/moderate vs poor/mucinous)	2.561	1.058-6.198	0.037
Tumor site
Left	1 (ref)
Right	0.735	0.205-2.636	0.637
Rectum	0.834	0.307-2.263	0.721
CEA (normal vs high)	4.722	1.933-11.535	0.001
CA19-9 (normal vs high)	3.961	1.633-9.606	0.002
MSI (MSS vs MSI)	0.047	0.000-1327.112	0.559
Lung metastasis (absent vs present)	2.325	0.844-6.405	0.103
Liver metastasis (absent vs present)	2.351	0.785-7.038	0.127
DCLK1 (low vs high)	3.958	1.496-10.472	0.006
Lgr5 (low vs high)	2.521	1.025-6.199	0.044
DCLK1/Lgr5 (others1 vs high/high)	3.808	1.471-9.854	0.006
Multivariate
Tumor stage (TNM) (II vs III)	6.846	1.918-24.445	0.003
Differentiation (well/moderate vs poor/mucinous)	1.864	0.699-4.971	0.241
CEA (normal vs high)	4.835	1.651-14.154	0.004
CA19-9 (normal vs high)	4.102	1.529-11.005	0.005
DCLK1 (low vs high)	4.656	1.207-17.956	0.026
Lgr5 (low vs high)	0.764	0.076-7.691	0.819
DCLK1/Lgr5 (others1 vs high/high)	3.429	0.212-55.583	0.386

¹Low/low low/high and high/low. HR: Hazard ratio; CI: Confidence interval; DCLK1: Doublecortin-like kinase 1; Lgr5: Leucine-rich repeat-containing G-protein-coupled receptor 5; TNM: Tumor-node-metastasis.