Herbal cake-partitioned moxibustion inhibits colonic autophagy in Crohn’s disease via signaling involving distinct classes of phosphatidylinositol 3-kinases

doi:10.3748/wjg.v26.i39.5997

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Oct 21, 2020; 26(39): 5997-6014
Published online Oct 21, 2020. doi: 10.3748/wjg.v26.i39.5997

Table 1 Primers used for PCR

NM	Length	Primer NM	Sequence (5’-3’)
NM_017008.4	129 bp	Rat GAPDH F-primer	tgccactcagaagactgtgg
		Rat GAPDH R-primer	ttcagctctgggatgacctt
NM_175843.4	188 bp	Rat p62 F-primer	agaagtggacccatccacag
		Rat p62 R-primer	agaaacccatggacagcatc
NM_022867.2	114 bp	Rat Map1lc3b F-primer	ttcttcctcctggtgaatgg
		Rat Map1lc3b R-primer	ctgggaggcatagaccatgt
NM_019906.1	153 bp	Rat mTOR Forward primer	ggcttctgaagatgctgtcc
		Rat mTOR Reverse primer	gagttcgaagggcaagagtg
NM_013005.1	166 bp	Rat Pik3r1 F-primer	actactggggagaggggaga
		Rat Pik3r1 R-primer	caggaagggtcaacactggt
NM_033230.2	176 bp	Rat Akt1 F-primer	actcattccagacccacgac
		Rat Akt1 R-primer	ccggtacaccacgttcttct

GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; p62: Sequestosome 1; Map1lc3b: Microtubule-associated protein 1 light chain 3 beta; mTOR: Mammalian target of rapamycin; Pik3r1: Phosphatidylinositol 3-kinase regulatory subunit alpha; Akt1: Protein kinase B akt-1.

Citation: Wang SY, Zhao JM, Zhou CL, Zheng HD, Huang Y, Zhao M, Zhang ZY, Wu LY, Wu HG, Liu HR. Herbal cake-partitioned moxibustion inhibits colonic autophagy in Crohn’s disease via signaling involving distinct classes of phosphatidylinositol 3-kinases. World J Gastroenterol 2020; 26(39): 5997-6014
URL: https://www.wjgnet.com/1007-9327/full/v26/i39/5997.htm
DOI: https://dx.doi.org/10.3748/wjg.v26.i39.5997