Arachidyl amido cholanoic acid improves liver glucose and lipid homeostasis in nonalcoholic steatohepatitis via AMPK and mTOR regulation

Figure 3 Effect of Arachidyl amido cholanoic acid on the proteome of cultured mouse hepatocytes. A total of 3220 proteins were identified by proteomics analysis, of which the contents of 219 changed significantly (6.80%, P < 0.05) between the two experimental groups [vehicle vs arachidyl amido cholanoic acid (Aramchol) 20 µmol/L]. A: Hierarchical clustering showing classification of the samples into two differentiated groups: Control and Aramchol treated; B: The differentially expressed proteins according to the up- or down-regulation were classified with colors in a volcano plot depending on their biological functions; C: Classification of the selected differentially expressed proteins in key biological functions. STRING software was used for the analysis.