Association between human leukocyte antigen gene polymorphisms and multiple EPIYA-C repeats in gastrointestinal disorders

Table 1 PCR primers

Primer	Primer sequence (59R39)	Ref.
cagA-F	GATAACAGGCAAGCTTTTGAGG1	[19,20]
cagA-R	CTGCAAAAGATTGTTTGGCAGA1
cagA28F	TTCTCAAAGGAGCAATTGGC2	[21]
cagA-P1C	GTCCTGCTTTCTTTTTATTAACTTKAGC2
cagA-P2CG	TTTAGCAACTTGAGCGTAAATGGG2
cagA-P2TA	TTTAGCAACTTGAGTATAAATGGG2
cagA-P3E	ATCAATTGTAGCGTAAATGGG2
“cag empty PCR”	GCTTGCTTGTATTGGCCTTG / GCATGCACATTCCCTAAAGT3	[22]

All polymerase chain reactions (PCRs) were performed in a 50 μL final volume with 1.25 U Taq polymerase, 16 μL PCR buffer, 3 μL 2.5 mM MgCl₂ and 1 μL dNTP mix (10 mM each) with the described primers using the thermal profiles detailed.

¹: 95 ºC for 2 min initial denaturation, followed by 45 cycles of 30 s at 95 ºC, 45 s at 53 ºC and 45 s at 72 ºC, with a final elongation step performed for 5 min at 72 ºC.

²: 95 ºC for 2 min initial denaturation, followed by 50 cycles of 30 s at 95 ºC, 45 s at 57 ºC and 35 s at 72 ºC, with a final elongation step performed for 5 min at 72 ºC.

³: 95 ºC for 2 min initial denaturation, followed by 40 cycles of 30 s at 95 ºC, 30 s at 57 ºC, 20 s at 72 ºC, with a final elongation step performed for 5 min at 72 ºC. PCR: Polymerase chain reaction.