Rno_circ_0005139 regulates apoptosis by targeting Wnt5a in rat anorectal malformations

Figure 5 Rno_circ_0005139 regulated cell proliferation and apoptosis via miR-324-3p/Wnt5a. A: Relative expression of Wnt5a was measured by quantitative reverse transcription polymerase chain reaction with si-rno_circ_0005139 (si-circ), pLO5-rno_circ_0005139 (over-circ), or a negative control (si-NC/vector); B: Wnt5a expression was measured by western blotting after co-transfection with si-rno_circ_0005139 (si-circ), miR-324-3p inhibitor, or a negative control (si-NC/inhibitor-NC), and co-transfection with pLO5-rno_circ_0005139 (over-circ), miR-324-3p mimic, or a negative control (over-NC/mimic-NC); C and D: A Cell Counting Kit-8 assay was used to measure viability in intestinal epithelial cells (IECs) after co-transfection (rno_circ_0005139 and miR-324-3p); E and F: An apoptosis assay with detection by flow cytometry was used to measure apoptosis in IECs after co-transfection (rno_circ_0005139 and miR-324-3p). ^aP < 0.05 vs si-circ_0005139-NC, ^bP < 0.05 vs over-circ_0005139-NC+miR-324-3p mimic NC, ^cP < 0.01 vs over-circ_0005139-NC+miR-324-3p mimic NC and ^dP < 0.01 vs over-circ_0005139-NC+miR-324-3p mimic.