Adipose-derived mesenchymal stem cells alleviate TNBS-induced colitis in rats by influencing intestinal epithelial cell regeneration, Wnt signaling, and T cell immunity

Figure 5 Changes in inflammatory markers and T cell-secreted molecules. A: Total mRNA from colonic extracts was analyzed by qRT-PCR for inflammatory cytokines involved in colitis: IFN-γ, IL-2, IL-17, IL-23, IL-4, IL-13, IL-10, and TGF-β; B: Western blot analysis of Foxp3, GATA3, RORγ, and T-bet. Data are presented as the mean ± SD. ^bP < 0.01 vs control group; ^cP < 0.05, ^dP < 0.01 vs CD group. IFN-γ: Interferon-γ; IL-6: Interleukin-6; IL-17: Interleukin-17; IL-23: Interleukin-23; IL-4: Interleukin-4; IL-13: Interleukin-13; IL-10: Interleukin-10; TGF-β: Transforming growth factor. ADSCs: Adipose-derived mesenchymal stem cells; CD: Crohn’s disease; GFP: Green fluorescent protein.