Adipose-derived mesenchymal stem cells alleviate TNBS-induced colitis in rats by influencing intestinal epithelial cell regeneration, Wnt signaling, and T cell immunity

Figure 3 Distribution of adipose-derived mesenchymal stem cells and their colocalization with intestinal stem cells and intestinal epithelial cells. A: Distribution of green fluorescent protein (GFP)-positive adipose-derived mesenchymal stem cells (ADSCs) was detected mostly in the inflamed colon (scale bar: 50 μm); B: Immunofluorescence analysis for GFP and CK-20 showed the colocalization between ADSCs and intestinal epithelial cells (scale bar: 50 μm); C: Immunofluorescence analysis for GFP and Lgr5 showed that there was no colocalization between ADSCs and intestinal stem cells (scale bar: 50 μm). ADSCs: adipose-derived mesenchymal stem cells; CD: Crohn’s disease; GFP: Green fluorescent protein.