Expression of Notch pathway components (Numb, Itch, and Siah-1) in colorectal tumors: A clinicopathological study

Table 3 Distribution of Notch-1, Numb, Itch, and Siah-1 expression levels assessed by qPCR analysis in adenoma and carcinoma groups

Gene expression levels	Adenomas	Carcinomas	P value
Notch
mean ± SD	3.1016 ± 1.0452	10.2628 ± 5.8043	0.03
Median	2.6992	1.5305
Normal (%)	0	12 (24)	0.0001
(↑) (%)	15 (50)	22 (44)
(↓) (%)	15 (50)	16 (32)
Numb
mean ± SD1	8.1362 ± 1.6299	2.3153 ± 2.2267	0.03
Median	4.0287	-2.2616
Normal (%)	4 (13.3)	0	0.0001
(↑) (%)	18 (60)	24 (58)
(↓) (%)	8 (26.7)	26 (52)
Itch
mean ± SD	9.2899 ± 2.7369	2.1982 ± 1.0461	0.006
Median	2.0422	1.5933
Normal (%)	11 (36.7)	12 (32)	0.0001
(↑) (%)	15 (50)	18 (38)
(↓) (%)	4 (13,3)	20 (30)
Siah-1
mean ± SD	5.9024 ± 1.18671	0.8358 ± 1.3831	0.03
median	1.4031	-2.0322
Normal (%)	6 (20)	10 (20)	0.001
(↑) (%)	11 (36.7)	13 (26)
(↓) (%)	13 (43.3)	27 (54)

¹Fold increase 2^-∆∆Ct. (↑): Upregulation; (↓): Downregulation.