Gan Shen Fu Fang ameliorates liver fibrosis in vitro and in vivo by inhibiting the inflammatory response and extracellular signal-regulated kinase phosphorylation

Figure 4 Gan Shen Fu Fang reduced extracellular signal-regulated kinase phosphorylation and NF-κB expression in common bile duct-ligated rats and HSC-T6 cells. A-D: Liver tissue homogenates were subjected to immunoblotting as indicated. Representative bans and quantitative data are shown. N = 4; E-G: HSC-T6 cells were not stimulated, and whole-cell lysates were used. Representative images and quantitative data are shown; H: HSC-T6 cells were stimulated with platelet-derived growth factor-BB to further evaluate the effect of GSFF on extracellular signal-regulated kinase phosphorylation. ^aP < 0.05, ^bP < 0.01, ^eP < 0.001, compared with control cells; ^cP < 0.05, ^dP < 0.01, ^fP < 0.001 compared with sham rats. ^gP < 0.05, ^hP < 0.01 compared with the 2W-CBDL rats; ⁱP < 0.05, ^lP < 0.001, compared with the 4W-CBDL rats; ⁿP <0.01, compared with cells stimulated with only platelet-derived growth factor-BB. PDGF: Platelet-derived growth factor; GSFF: Gan Shen Fu Fang; ERK: Extracellular signal-regulated kinase; CBDL: Common bile duct-ligated.