Gan Shen Fu Fang ameliorates liver fibrosis in vitro and in vivo by inhibiting the inflammatory response and extracellular signal-regulated kinase phosphorylation

Figure 3 Gan Shen Fu Fang inhibited HSC-T6 cell viability and collagen synthesis. A and B: GSFF inhibited HSC-T6 cell viability and Col. I release; C and D: GSFF inhibited the viability and Col. I release of HSC-T6 cells stimulated with transforming growth factor β1 (TGF-β1); E and F: TGF-β1 promoted α-SMA expression in HSC-T6 cells, which was decreased by GSFF. The relative α-SMA content was measured by quantitative histomorphometry, and the results are shown in panel E. ^aP < 0.05, ^bP < 0.01, compared with the control cells. ^cP < 0.05, ^dP < 0.01, ^fP < 0.001, compared with cells stimulated with TGF-β1 only. GSFF: Gan Shen Fu Fang; TGF-β1: Transforming growth factor β1.