Decreased of BAFF-R expression and B cells maturation in patients with hepatitis B virus-related hepatocellular carcinoma

Figure 1 Representative plot shows the gating strategy for identification of B cell subsets. (1) Firstly, lymphocyte population was initially determined from forward scatter and side scatter plot. (2) Positive for CD19 was selected from CD19 vs CD27 plot. (3) Naive B cells, IgD and class-switched memory B cells were then identified from IgD vs CD27 plot. And (4) Plasmablasts and transitional B cells were selected from CD38 vs CD27 and CD38 vs CD24, respectively. Moreover, B cell-activating factor receptors were gated on CD19 positive cells. FSC: Forward scatter; SSC: Side scatter; BAFF-R: B cell-activating factor receptors; BCMA: B-cell maturation antigen; TACI: Transmembrane activator and cyclophilin ligand interactor.