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©The Author(s) 2020.
World J Gastroenterol. May 28, 2020; 26(20): 2599-2617
Published online May 28, 2020. doi: 10.3748/wjg.v26.i20.2599
Published online May 28, 2020. doi: 10.3748/wjg.v26.i20.2599
Table 1 Specific sequences of primers used in polymerase chain reaction analysis
| Gene | Primer sequence, 5’-3’ | GenBank No. | Length, bp | Annealing temperature, °C |
| GAPDH | F: CTGGAGAAACCTGCCAAGTATG | NM_017008.4 | 138 | 60 |
| R: GGTGGAAGAATGGGAGTTGCT | ||||
| ET-1 | F: TCTGCCACCTGGACATCATCTG | NM_012548.2 | 205 | 60 |
| R: CTTTGGGCTCGGAGTTCTTTGT | ||||
| ET-A | F: ACCGCCATTGAAATTGTCTCC | NM_012550.2 | 173 | 60 |
| R: AGCCACCAGTCCTTCACGTCTT | ||||
| ET-B | F: CCAAAGACTGGTGGCTGTTCA | XM_006252431.3 | 183 | 60 |
| R: CAAACACGAGGACCAGGCAG | ||||
| ECE-1 | F: CACAACCAAGCCATCATTAAGC | NM_053596.2 | 275 | 60 |
| R: TTGGAGTCGGCACTGACATAGA |
- Citation: Ruze R, Xiong YC, Li JW, Zhong MW, Xu Q, Yan ZB, Zhu JK, Cheng YG, Hu SY, Zhang GY. Sleeve gastrectomy ameliorates endothelial function and prevents lung cancer by normalizing endothelin-1 axis in obese and diabetic rats. World J Gastroenterol 2020; 26(20): 2599-2617
- URL: https://www.wjgnet.com/1007-9327/full/v26/i20/2599.htm
- DOI: https://dx.doi.org/10.3748/wjg.v26.i20.2599