MiR-301a transcriptionally activated by HIF-2α promotes hypoxia-induced epithelial-mesenchymal transition by targeting TP63 in pancreatic cancer

Figure 5 MiR-301a directly targets TP63 in pancreatic cancer. A: Prediction of miR-301a binding to the 3’ untranslated region (3’UTR) of TTP; B: Luciferase reporter assays confirmed that P63 was a miR-301a target gene; C: Immunofluorescence was performed to detect the expression of P63 in miR-301a-upregulated cell lines under normoxia and hypoxia; D and E: Western blot was performed to detect the expression of P63 protein after the overexpression and knockout of miR-301a; G: The expression of TP63 and the epithelial-mesenchymal transition markers were detected in the miR-301a-upregulated BxPC-3 cell line by Western blot, and (F) Transwell assays were performed to measure cell migration; H: TP63 was knocked down in the miR-301a knockout cell line PANC-1, and (F) Transwell assays were performed to measure cell migration. ^bP < 0.01, ^cP < 0.001.