MiR-301a transcriptionally activated by HIF-2α promotes hypoxia-induced epithelial-mesenchymal transition by targeting TP63 in pancreatic cancer

Figure 4 Knockdown of HIF-1α or HIF-2α by siRNA interference and detection of miR-301a expression by qRT-PCR. A and B: After cells were transfected with siRNA, real-time PCR was performed to measure the mRNA expression levels of hypoxia-inducible factor (HIF)-1α and HIF-2α in CFPAC-1 cells; C and D: Real-time RCR was performed to measure miR-301a expression after the knockdown of HIF-1α and HIF-2α; E: HIF-1α significantly promoted luciferase activity in the positive control group (HRE-Luc) but had no effect on the activity of the miR-301a promoter; F: Transfections with different doses of the HIF-2α plasmids significantly promoted miR-301a promoter activity in a dose-dependent manner; G: Transfection with HIF-2α significantly promoted the transcriptional activity of the truncated No. 1 miR-301a promoter in a dose-dependent manner but had no effect on the truncated No. 2 and No. 3 promoters. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. HIF: Hypoxia-inducible factor.