MiR-301a transcriptionally activated by HIF-2α promotes hypoxia-induced epithelial-mesenchymal transition by targeting TP63 in pancreatic cancer

Figure 3 MiR-301a knockout results in the suppression of hypoxia-induced epithelial-mesenchymal transition. A: To identify miR-301a knockout cell lines, the amplification product was tested by gel electrophoresis after amplifying the miR-301a flanking sequence; B: Sanger sequencing showed that the No. 2 monoclonal cell line had 169 bases, including the miR-301a sequence, deleted from the genome; C: qRT-PCR was used to measure miR-301a expression in PANC-1 cells after miR-301a knockout; D: HIF-1α was downregulated in the miR-301a knockout cells cultured under hypoxia for 48 h; miR-301a knockout in PANC-1 cells promoted the expression of the epithelial markers (E-cadherin and ZO-1) and inhibited the expression of the mesenchymal markers (Fibronectin and Vimentin); E: The mesenchymal morphological changes of the pancreatic cancer cells under hypoxia were blocked after miR-301a was knocked out; F and G: Transwell assays and wound healing assays showed that PANC-1 cell migration was suppressed after miR-301a was knocked out. ^cP < 0.001.