Folic acid attenuates high-fat diet-induced steatohepatitis via deacetylase SIRT1-dependent restoration of PPARα

Figure 4 Folic acid restores the expression levels of PPARα via SIRT1 in rats with high-fat diet-induced steatohepatitis and Huh7 cell line. A-C: The expression levels of SIRT1 and PPARα in each group of rats; D, F, and G: The expression levels of SIRT1 and PPARα in Huh7 cell line exposed to 0.3 mmol/L PA; E, H, and I: The expression levels of SIRT1 and PPARα in Huh7 cell line transfected with SIRT1 siRNA and then exposed to 0.3 mmol/L PA. All the data are expressed as the mean ± SE (n = 3). ^aP < 0.05 vs con group; ^bP< 0.01 vs con group; ^dP < 0.01 vs HFD group; ^eP < 0.05 vs control; ^fP < 0.05 vs 0.3 PA group; ^gP < 0.01 vs 0.3 PA group; ^hP < 0.01 vs SIRT1-NC group; ⁱP < 0.05 vs 0.3 PA group; ^jP < 0.01 vs 0.3PA group; ^kP <0.01 vs 5-MTHF and 0.3 PA group. HFD: High-fat diet; PPARα: Peroxisome proliferator-activated receptor alpha; SIRT1: Silence information regulation factor 1; PA: Palmitic acid; 5-MTHF: 5-methyltetrahydrofolic acid.