PTEN-induced kinase 1-induced dynamin-related protein 1 Ser637 phosphorylation reduces mitochondrial fission and protects against intestinal ischemia reperfusion injury

Figure 7 PTEN-induced kinase 1 inhibits dynamin-related protein 1 recruitment on mitochondria by binding to Ser637. A, C: Mitochondrial localization of Dynamin-related protein 1 (DRP1) assessed under confocal a laser scanning microscope (green). Mitochondrial morphology was stained with MitoTracker Deep Red FM (red), and nuclei were stained with DAPI (blue) (scale bar = 10 μm); B, D: Representative Western blotting of DRP1 Ser637 protein expression in Caco-2 cells (n = 3); E: Coimmunoprecipitation assay of PTEN-induced kinase 1 with DRP1 Ser637. Representative immunoblot revealing the interaction of DRP1 Ser637 with PTEN-induced kinase 1. The input represents the total protein extracts used in immunoprecipitation. IB, immunoblotting; IP, immunoprecipitation, IgG, negative control. ^bP < 0.01 compared with the control group; ^dP < 0.01 compared with the H/R group. DRP1: Dynamin-related protein 1; PINK1: PTEN-induced kinase 1; H/R: hypoxia/reoxygenation; DAPI: 4',6-diamidino-2-phenylindole; IB: Immunoblot; IP: Immunoprecipitation.