PTEN-induced kinase 1-induced dynamin-related protein 1 Ser637 phosphorylation reduces mitochondrial fission and protects against intestinal ischemia reperfusion injury

Figure 6 Promotion of PTEN-induced kinase 1 expression decreases mitochondrial fission, reactive oxygen species accumulation and apoptosis in Caco-2 hypoxia/reoxygenation cells. A: Representative Western blotting of PTEN-induced kinase 1 (PINK1) protein expression in Caco-2 cells (n = 3); B: PINK1 overexpression increased Caco-2 cell viability according to CCK-8 assay (n = 6); C: PINK1 overexpression decreases mitochondrial fission during Hypoxia/reoxygenation (H/R) in Caco-2 cells according to MitoTracker staining (scale bars = 10 μm); D: PINK1 overexpression decreases reactive oxygen species levels detected by MitoSOX during H/R in Caco-2 cells (scale bars = 10 μm) (n = 3); E: TUNEL assay showing that PINK1 overexpression decreases apoptosis (scale bar = 100 μm); F: PINK1 overexpression decreases apoptosis, asindicated by the TUNEL-positive cell ratio (n = 3). ^aP < 0.05, ^bP < 0.01, ^cP < 0.001 compared with the control group; ^eP < 0.01 compared with the H/R group. PINK1: PTEN induced kinase 1; ROS: Reactive oxygen species; CCK-8: Cell Counting Kit-8; H/R: Hypoxia/reoxygenation; TUNEL: Terminal-deoxynucleoitidyl Transferase Mediated Nick End Labeling; DAPI: 4',6-diamidino-2-phenylindole.