PTEN-induced kinase 1-induced dynamin-related protein 1 Ser637 phosphorylation reduces mitochondrial fission and protects against intestinal ischemia reperfusion injury

Figure 3 Mdivi-1 treatment protects cell viability and decreases apoptosis in Caco-2 hypoxia/reoxygenation cells. A: Cell viability was detected by the CCK8 method (n = 6); B: Representative Western blotting of cleaved caspase-3 protein expression in Caco-2 cells (n = 3); C: Effect of mdivi-1 on hypoxia/reoxygenation-induced cell apoptosis detected by TUNEL assay (scale bar = 100 μm); D: TUNEL positive cell ratio in hypoxia/reoxygenation-induced cell apoptosis (n = 3). ^aP < 0.05, ^bP < 0.01, ^cP < 0.001 compared with the control group; ^dP < 0.05, ^eP < 0.01 compared with the hypoxia/reoxygenation group. c-caspase 3: Cleaved caspase 3; H/R: Hypoxia/reoxygenation; TUNEL: Terminal-deoxynucleotidyl Transferase Mediated Nick End Labeling; DAPI: 4',6-diamidino-2-phenylindole.