Effects of long non-coding RNA Opa-interacting protein 5 antisense RNA 1 on colon cancer cell resistance to oxaliplatin and its regulation of microRNA-137

Figure 6 Effects of Opa-interacting protein 5 antisense RNA 1 on the expression of miR-137 in oxaliplatin-resistant colon cancer cell lines. A: Binding sites between OIP 5-AS1 and miR-137 and dual luciferase reporter assay; B: Verification of the relation between Opa-interacting protein 5 antisense RNA 1 and miR-137 by the RIP assay; C: The miR-137 expression in drug-resistant cell lines after co-transfection; D: Growth inhibition of HCT116/oxaliplatin (L-OHP) cell lines after co-transfection; E: Growth inhibition of SW480/L-OHP cell lines after co-transfection; F: Comparison of apoptosis rate of cell lines among different groups after co-transfection; G: Comparison of apoptosis protein level in drug resistant cell lines among different groups after co-transfection; H: Apoptosis of drug-resistant cell lines in each group after co-transfection; I: Apoptosis-related protein in drug-resistant cell lines in each group after co-transfection. SW480/L-OHP indicates SW480 cell line with L-OHP resistance. HCT116/L-OHP indicates HCT116 cell line with L-OHP resistance. ^bP < 0.01, ^eP < 0.001 for between-group comparisons. L-OHP: Oxaliplatin; miR-NC: MiR negative control; Wt: Wild type; Mut: Mutant.