Calpain-2 activity promotes aberrant endoplasmic reticulum stress-related apoptosis in hepatocytes

Figure 4 Dithiothreitol increases the levels of intracellular Ca²⁺ in BRL-3A cells. After treatment with 2.0 mmol/L dithiothreitol for varying time periods, the cells were labeled by Fluo-3 AM. The cell morphology and fluorescent signals in cells were observed by microscopy. A: Representative images of Fluo-3 fluorescence and morphology in BRL-3A cells following dithiothreitol treatment (Scale bars: 25 μm); B: Quantification of fluorescence intensity in BRL-3A cells. Data are representative images (magnification 200 ×) or expressed as the mean ± SD of each group of cells from three separate experiments. ^bP < 0.01.