Abdominal paracentesis drainage ameliorates myocardial injury in severe experimental pancreatitis rats through suppressing oxidative stress

Figure 7 Effects of pancreatitis associated ascitic fluids intraperitoneal injection with or without anti-high mobility group box 1 neutralizing antibody on cardiac NOX expression in cerulein rats. A: High mobility group box 1 in the serum; B: Nicotinamide adenine dinucleotide phosphate oxidase-2 (NOX2) and NOX4 mRNA measurement by real-time polymerase chain reaction; C: Immunoblot of NOX2 and NOX4 protein expression from heart samples; D: Densitometry analysis of NOX2 and NOX4. Data indicate the mean ± standard deviation obtained from six animals in each group (A-B). Data are representative of at least three independent experiments (C-D). ^aP < 0.05 vs controls group; ^cP < 0.05 vs pancreatitis-associated ascitic fluid injection group. HMGB1: High mobility group box 1; NOX2: Nicotinamide adenine dinucleotide phosphate oxidase-2; NOX4: Nicotinamide adenine dinucleotide phosphate oxidase-4; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; SAP: Severe acute pancreatitis; APD: Abdominal paracentesis drainage.