Abdominal paracentesis drainage ameliorates myocardial injury in severe experimental pancreatitis rats through suppressing oxidative stress

Figure 5 Abdominal paracentesis drainage attenuates myocardial cell apoptosis and expression of apoptosis associated protein in myocardium. A: Representative images (400 × magnification) of terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay. Cells with green staining in the nuclei were determined to be apoptotic cells; B: Immunoblot of Bax and Bcl-2 protein expression from heart samples; C: Densitometry analysis of Bax and Bcl-2. Data are representative of at least three independent experiments; D: Immunoblot of pro-caspase-3 and cleaved-caspase-3 protein expression from heart samples; E: Densitometry analysis of pro-caspase-3 and cleaved-caspase-3. Data are representative of at least three independent experiments. ^aP < 0.05 vs sham group; ^cP < 0.05 vs severe acute pancreatitis group. TUNEL: Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling; Bax: Bcl-2 related X protein; Bcl-2: B cell lymphoma/leukemia-2 gene; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; SAP: Severe acute pancreatitis; APD: Abdominal paracentesis drainage.