Abdominal paracentesis drainage ameliorates myocardial injury in severe experimental pancreatitis rats through suppressing oxidative stress

Figure 4 Effect of abdominal paracentesis drainage on the protein expression and activity of nicotinamide adenine dinucleotide phosphate oxidase, reactive oxygen species production and oxidative indexes. A: Immunoblot of nicotinamide adenine dinucleotide phosphate oxidase-2 (NOX2) and NOX4 protein expression from heart samples; B: Densitometry analysis of NOX2 and NOX4; C: NOX activity was measured by the colorimetric method in heart tissues; D: Dihydroethidium staining in frozen sections by fluorescent microscopy (100 × magnification); E: The fluorescence values are expressed as the ratio to the levels in the sham group; F-H: The levels of lipid peroxidation, the activity of superoxide dismutase and reduced glutathione in heart tissues, respectively. Data are representative of at least three independent experiments (A-B). Data indicate the mean ± standard deviation obtained from six animals in each group (C-H). ^aP < 0.05 vs sham group; ^cP < 0.05 vs severe acute pancreatitis group. NOX2: Nicotinamide adenine dinucleotide phosphate oxidase-2; NOX4: Nicotinamide adenine dinucleotide phosphate oxidase-4; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; SAP: Severe acute pancreatitis; APD: Abdominal paracentesis drainage; ROS: Reactive oxygen species; LPO: Lipid peroxidation; SOD: Superoxide dismutase; GSH: Reduced glutathione.