Feedback regulation between phosphatidylinositol-3,4,5-trisphosphate dependent Rac exchange factor 1 and transforming growth factor β1 and prognostic value in gastric cancer

Figure 5 Feedback regulation between phosphatidylinositol-3,4,5-trisphosphate dependent Rac exchange factor 1 and transforming growth factor β1 in gastric cancer cells. A and B: SGC-7901 cells and BGC-823 cells were treated with recombinant human transforming growth factor (TGF) β1 protein at the concentration of 20 ng/mL for 12 h, the mRNA level was assessed by reverse transcriptase quantitative polymerase chain reaction; C: SGC-7901 and BGC-823 cells were treated with recombinant human TGFβ1 protein at the concentration of 20 ng/mL for 12 h, the phosphatidylinositol-3,4,5-trisphosphate dependent Rac exchange factor 1 (PREX1) protein was examined by western blotting; D and E: SGC-7901 and BGC-823 cells were cultured with lentivirus of PREX1 overexpressing particle in dose concentration and control for 24 h, the cells were subjected to western blotting to evaluate the activation of TGFβ1; F: HEK293T cells were transfected with Luc-PT3 and Rellina plasmids, the culture medium was supplemented with lentivirus particle of PREX1 overexpression and vector control. For another 24 h culture, the cells were treated with recombinant TGFβ1 (20 ng/mL) for 6 h, then the cells were subjected to dual-luciferase reporter assay. Statistical significance is expressed as ^aP < 0.05, ^bP < 0.01, ^eP < 0.001. PREX1: Phosphatidylinositol-3,4,5-trisphosphate dependent Rac exchange factor 1; TGFβ1: Transforming growth factor β1.