Reaction of antibodies to Campylobacter jejuni and cytolethal distending toxin B with tissues and food antigens

Figure 1 Reaction of mouse monoclonal anti-Campylobacter jejuni with tissue antigens. Compared to the monoclonal antibody’s reaction with Campylobacter jejuni (C. jejuni) lysate as positive control and human serum albumin (HSA) or unimmunized mouse serum as negative control, the reaction of this antibody with 15 tissue antigens is insignificant, with 25 additional antigens is low, with β-amyloid, zonulin, acetylcholine receptor, somatotropin and presenilin ranges from moderate to high, and with C. jejuni lysate is very highly positive. The variation of quadruplicate ODs was less than 10% for all determinations. Comparison of the means of the antibody reactivity to various tissue antigens with the means of controls resulted in insignificant values (P > 0.05) for 15 different tissue antigens and highly significant P values (P < 0.00001) for the others. 0-0.4 OD = insignificant; 0.42-1.0 = low; 1.1-1.6 = moderate; 1.61-2.2 = high; > 2.21 = very high. tTG: Transglutaminase; mTG: Microbial transglutaminase; DPPIV: Dipeptidyl peptidase-4; TPO: Thyroid peroxidase; PDI: Protein disulfide isomerase; GFAP: Glial fibrillary acidic protein; H. AQP4: Human aquaporin-4; MBP: Myelin basic protein; HSA: Human serum albumin.