Dynamic changes of key metabolites during liver fibrosis in rats

Figure 4 Metabolomic profiling and flux analyses. A: Principle component analysis for the control and fibrosis model groups at weeks 1, 4, 8, and 12; B: Orthogonal partial least squares discriminant analysis score plots for the control and fibrosis model groups at weeks 1, 4, 8, and 12; C: Heat map generated from the liquid chromatography-mass spectrometry data using the hierarchical clustering algorithm; D-G: Volcano plot analyses were used to determine the significant metabolites in the fibrosis model groups compared to controls at weeks 1, 4, 8, and 12. Data points with fold changes > 2 and P < 0.05 are labeled in red.