Dynamic changes of key metabolites during liver fibrosis in rats

Figure 3 Immunohistochemistry results and relative mRNA expression levels of α-smooth muscle actin and transforming growth factor β1. A-E: Immunohistochemical staining for α-SMA; F: qRT-PCR results of α-SMA at each time point; G-K: Immunohistochemical staining for TGF-β1; L: qRT-PCR results of TGF-β1 at each time point. The data are presented as the mean ± SD (error bars) and were statistically analyzed using a Student’s t-test. ^aP < 0.01 vs control. Scale bars: 100 μm. α-SMA: α-smooth muscle actin; TGF-β1: Transforming growth factor-β1; qRT-PCR: Quantitative real-time polymerase chain reaction.