Ubiquitin-specific protease 22 enhances intestinal cell proliferation and tissue regeneration after intestinal ischemia reperfusion injury

Figure 2 Ubiquitin-specific protease 22 correlates with the proliferative activity of IEC-6 cells after hypoxia/reoxygenation. IEC-6 cells were subjected to 6 h of hypoxia followed by 3, 6, 12, or 24 h of reoxygenation. A: Representative Western blot demonstrating the expression of USP22 in tissues from the experimental groups (n = 3); B: Representative Western blot demonstrating the expression of Cyclin D1 in tissues from the experimental groups (n = 3); C: CCK-8 was used to examine cell proliferation and viability at the indicated time points (n = 6). The results are presented as the mean ± SD. ^aP < 0.05 vs the control group; ^bP < 0.01 vs the control group. USP22: Ubiquitin-specific protease 22; CCK-8: Cell Counting Kit-8.