Ubiquitin-specific protease 22 enhances intestinal cell proliferation and tissue regeneration after intestinal ischemia reperfusion injury

Figure 1 Ubiquitin-specific protease 22 is positively correlated with cell proliferation and tissue regeneration after ischemia reperfusion injury. Anesthetized rats were subjected to 60 min of intestinal ischemia followed by 3, 6, 12, or 24 h of reperfusion and tissues were harvested at the end of reperfusion. A: Intestinal tissue sections of the experimental groups were stained with HE (200×, bars represent 100 μm); B: Intestinal tissues in injury was scored histopathologically according to a scoring system (Chiu’s score); C: Representative Western blot demonstrating the expression of USP22 in tissues from the experimental groups (n = 3); D: Immunohistochemical staining for PCNA was used for intestinal tissue sections of the experimental groups (400×, bars represent 50 μm); E: Quantitative analyses for immunohistochemical staining. The arrows show brown positive nuclei of proliferating intestinal cells in the crypt area of the intestinal gland; F: Representative protein levels of Cyclin D1 (n = 3) were demonstrated by Western blot. The results are presented as the mean ± SD. ^bP < 0.01 vs sham. USP22: Ubiquitin-specific protease 22; PCNA: Proliferating cell nuclear antigen.