Claudin-7 gene knockout causes destruction of intestinal structure and animal death in mice

Figure 2 Long range PCR and Southern blot for detecting target clones. A: The 5’ homologous arm band was first detected, and the product with a length of 5496 bp was a positive clone containing LoxP; B: The 3’ end was then detected, and the product with a length of 5204 bp was a positive clone; C: Southern blot results showing that clones 8D, 8E, 4F, 11E, and 11F were the final targeted clones; D: After blastocyst transfer, newborn mice were genotyped by PCR. The mice numbered 50 and 77 were deemed chimeric mice with the genotype fln/wt.