Effect of adipose-derived mesenchymal stem cells on hepatocellular carcinoma: In vitro inhibition of carcinogenesis

Figure 3 Expression of hepatocellular markers in hepatocellular carcinoma cells. HCC cells (2 × 10⁵) were seeded in six-well coculture plates in the presence or absence of ADMSCs and ADMSC CM, undiluted or diluted 1:5 or 1:25 for 48 h. The protein levels of AFP (A) and DCP (C) was measured in the cell supernatant by ELISA; B: AFP mRNA was assessed by quantitative PCR. Results re displayed as percentage of controls. Data are represented as mean ± SD of five independent experiments, each repeated in triplicate (^aP < 0.05, ^bP < 0.01, ^cP < 0.001). CTR: Control; ADMSC: Adipose-derived mesenchymal stem cell; CM: Conditioned media; HCC: Hepatocellular carcinoma; AFP: alpha-fetoprotein; DCP: Des-gamma-carboxyprothrombin.