Claudin-7 gene knockout causes destruction of intestinal structure and animal death in mice

doi:10.3748/wjg.v25.i5.584

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Feb 7, 2019; 25(5): 584-599
Published online Feb 7, 2019. doi: 10.3748/wjg.v25.i5.584

Table 1 PCR primers for chimeric mouse genotyping and corresponding band results

Sequence (5’-3’)	Results
Cldn7 loxP-tF primer : GTACGAGTTTGGACCT GCCA	Fln/Fln = 493 bp; Fln/wt = 493/375 bp; wt/wt = 375 bp
Cldn7-LoxP-tR primer : TGTGCAA GGATCTGGGTCTG	Fln/Fln = 493 bp; Fln/wt = 493/375 bp; wt/wt = 375 bp
Cldn7-FRT-tF primer : CTGATCTGGGTGTCCCACGT	Fln/Fln = 507 bp; Fln/wt = 507/0 bp; wt/wt = 0bp
ZMK-2F4 primer: GCATCGCATTGTCTGAG TAGGTG	Fln/Fln = 507 bp; Fln/wt = 507/0 bp; wt/wt = 0bp
Neo-5R primer : GGCTGG ACGTAAACTCCTC	Fln/Fln = 259 bp; Fln/wt = 259/0bp; wt/wt = 0 bp
Cldn7-FRT-tR primer: CGATGAAACC GTTCCAGGTA	Fln/Fln = 259 bp; Fln/wt = 259/0bp; wt/wt = 0 bp

Citation: Xu C, Wang K, Ding YH, Li WJ, Ding L. Claudin-7 gene knockout causes destruction of intestinal structure and animal death in mice. World J Gastroenterol 2019; 25(5): 584-599
URL: https://www.wjgnet.com/1007-9327/full/v25/i5/584.htm
DOI: https://dx.doi.org/10.3748/wjg.v25.i5.584