Significance of postoperative follow-up of patients with metastatic colorectal cancer using circulating tumor DNA

Table 1 Characteristics of the markers used for the mutation analysis[16,17]

Marker	Exon number	Target codons	Size of PCR product (bp)	LOD (%)	DCE separation temperature (°C)
KRAS	2	12, 13	112	0.03	50
	3	59, 61	100	0.05	51
	4	117, 146	150	0.05	45
TP53	5	170-187	107	0.1	58
	6	187-224	169	0.5	52
	7	225-261	160	0.5	52
	8	262-307	151	0.03	56
APC	15	854-896	128	0.7	48
	15	1275-1308	100	0.7	48
	15	1290-1335	136	0.6	52
	15	1389-1446	174	0.3	48
	15	1430-1463	101	1	48
	15	1479-1530	156	1	51
	15	1539-1585	141	0.8	52
PIK3CA	9	542	106	0.2	48
	20	1025, 1031, 1047	136	0.3	49
BRAF	15	600	230	0.05	48
CTNNB1	3	45	152	0.4	52

bp: Base pair; DCE: Denaturing capillary electrophoresis; LOD: Limit of detection.