Aberrant methylation of secreted protein acidic and rich in cysteine gene and its significance in gastric cancer

Figure 1 Expression of secreted protein acidic and rich in cysteine in gastric cancer cell lines and eight pairs of samples. A: Specific reverse transcriptase quantitative real-time PCR analyzed for secreted protein acidic and rich in cysteine (SPARC). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as loading control. Relative SPARC mRNA expression levels were analyzed by densitometry followed by quantitation relative to GAPDH; B: Western blot analysis used a rabbit monoclonal SPARC antibody (43 kDa), GAPDH was used as control (34 kDa); C: Western blot analyzed SPARC gene expression in eight cases of gastric cancer samples, and GAPDH was used as internal reference. Expression level data are presented as mean ± SE. ^aP < 0.05 was considered statistically significant. SPARC: Secreted protein acidic and rich in cysteine; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; N: Non-tumor tissue; T: Tumor tissue.