LB100 ameliorates nonalcoholic fatty liver disease via the AMPK/Sirt1 pathway

Figure 3 LB100 inhibits serine/threonine protein phosphatase 2A activity and decreases free fatty acid-induced lipid accumulation in L02 cells. Free fatty acid (1 mmol/L)-stimulated L02 cells were treated with LB100 (6 μmol/L) or vehicle for 24 h. A: Serine/threonine protein phosphatase 2A (PP2A) activity tested by a commercial assay (upper), protein expression of methylated PP2A and PP2A C subunit (lower) of L02 cells in the different groups; B: Intracellular triglyceride content in L02 cells; C: Representative image of oil red O staining of L02 cells in the different groups. Scale bar in panel represents 100 μm. Data are presented as the mean ± standard deviation. ^aP < 0.05, ^bP < 0.01 vs bovine serum albumin group; ^cP < 0.05, ^dP < 0.01 vs FFA group. FFA: Free fatty acid; PP2A: Serine/threonine protein phosphatase 2A; TG: Triglyceride; BSA: Bovine serum albumin.