Long noncoding RNA NALT1-induced gastric cancer invasion and metastasis via NOTCH signaling pathway

Figure 5 NALT1 influence NOTCH1 expression. A: Schematic figure of NALT1 and NOTCH1 in chromosome 9. They were highlight with red box; B: NOTCH1 was overexpression in GC cells by RT-PCR; C: NOTCH1 was overexpression in GC cells by western-blot; D: The expression of NOTCH1 mRNA was effected by NALT1; E: The expression of protein was effected by NALT1; F: Transwell assay was used to evaluate the association of NALT1 and NOTCH1 in invasion in NALT1-knockdown and NALT1-knockdown + NOTCH1-overexpression GC cells; G: Scrape motility assays were monitored for 24 h in NALT1-knockdown and NALT1-knockdown + NOTCH1-overexpression GC cells. U6 and β-actin were used as loading control in RT-PCR and western-blot, respectively. In all figures, 100 × magnification was used. n = 3, ^aP < 0.05, ^bP < 0.01. NALT1: NOTCH1 associated with lncRNA in T cell acute lymphoblastic leukemia 1; GC: Gastric cancer.