Reduced microRNA 375 in colorectal cancer upregulates metadherin-mediated signaling

Figure 4 MicroRNA 375 regulates metadherin-mediated NFKB1 signaling in colorectal cancer cell lines. Western blot analysis of NF-κB inhibitor alpha (NFKBIA), NFKB1 (p50), and RELA (p65) expression levels in colorectal cancer (CRC) cells. A: NFKBIA expression levels were upregulated on microRNA 375 (MIR375) mimic and siMTDH transfection in HCT116 cells; B: NFKBIA expression levels were upregulated on MIR375 mimic and siMTDH transfection in Caco2 cells; C: NFKB1 and RELA levels were downregulated on MIR375 mimic and siMTDH transfection in HCT116 cells; D: NFKB1 and RELA levels were downregulated on MIR375 mimic and siMTDH transfection in Caco2 cells. Four independent experiments were performed with duplicates and P values were calculated using Student’s t-test (^aP < 0.05, ^bP < 0.01). MTDH: Metadherin; MIR375: MicroRNA 375; NFKBIA: NF-κB inhibitor alpha; CRC: Colorectal cancer.