Reduced microRNA 375 in colorectal cancer upregulates metadherin-mediated signaling

Figure 3 MicroRNA 375 regulates metadherin-mediated phosphatidylinositol-4,5-biphosphate-3-kinase catalytic subunit alpha-protein kinase B signaling in colorectal cancer cell lines. A: Western blot analysis of phosphatidylinositol-4,5-biphosphate-3-kinase catalytic subunit alpha (PIK3CA), protein kinase B (AKT), pAKT, and vascular endothelial growth factor A (VEGFA) expression levels in colorectal cancer (CRC) cells; B: Western blot analysis of PIK3CA, AKT, pAKT, and VEGFA expression levels in CRC cells; C: PIK3CA, AKT, pAKT, and VEGFA expression levels were downregulated on microRNA 375 (MIR375) mimic and siMTDH transfection in HCT116 cells; D: PIK3CA, AKT, pAKT, and VEGFA expression levels were downregulated on MIR375 mimic and siMTDH transfection in Caco2 cells. Five independent experiments were performed with duplicates and P values were calculated using Student’s t-test (^aP < 0.05, ^bP < 0.01, ^cP < 0.001). MTDH: Metadherin; PIK3CA: Phosphatidylinositol-4,5-biphosphate-3-kinase catalytic subunit alpha; AKT: Protein kinase B; VEGFA: Vascular endothelial growth factor A; MIR375: microRNA 375; CRC: Colorectal cancer.