Ubiquitin-conjugating enzyme E2T knockdown suppresses hepatocellular tumorigenesis via inducing cell cycle arrest and apoptosis

Figure 6 Dys-regulated genes in SMMC-7721 cells with ubiquitin-conjugating enzyme E2T knockdown. A: GeneChip assay showed that a total of 630 genes were regulated by ubiquitin-conjugating enzyme E2T (UBE2T) knockdown in SMMC-7721 cells (354 genes were upregulated and 276 genes downregulated) (P < 0.05, fold change > 2); B: The pathway enrichment analysis was performed using IPA software. Orange indicates activated pathways, and blue indicates suppressed pathways; C: Possible regulation network for UBE2T. The map shows upregulated SOD2 and its potential downstream targets. Pink box indicates upregulated genes, and green box indicates downregulated genes; D: qRT-PCR analysis of the indicated genes in ShCtrl and ShUBE2T SMMC-7721 cells. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001; E: ShCtrl and ShUBE2T SMMC-7721 cells were subjected to Western blot analysis.