Ubiquitin-conjugating enzyme E2T knockdown suppresses hepatocellular tumorigenesis via inducing cell cycle arrest and apoptosis

Figure 2 Ubiquitin-conjugating enzyme E2T knockdown reduces the viability of hepatocellular carcinoma cells. A and B: ShCtrl or ShUBE2T SMMC-7721 cells were subjected to qRT-PCR and Western blot analyses of UBE2T. GAPDH served as an internal control. ^bP < 0.01; C and D: ShCtrl or ShUBE2T BEL-7404 cells were subjected to qRT-PCR and Western blot analyses of UBE2T. GAPDH served as an internal control. ^bP < 0.01; E and F: ShCtrl or ShUBE2T SMMC-7721 cells were subjected to multiparametric high-content screening (HCS) analysis of cell viability from day 1 to day 5. Representative images of HCS and quantification of HCS. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001; G and H: ShCtrl or ShUBE2T BEL-7404 cells were subjected to multiparametric HCS analysis of cell viability from day 1 to day 5; G: Representative images of HCS; H: Quantification of HCS. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. UBE2T: Ubiquitin-conjugating enzyme E2T.