MicroRNA-30c inhibits pancreatic cancer cell proliferation by targeting twinfilin 1 and indicates a poor prognosis

Figure 3 Twinfilin 1 is a direct target of miR-30c. A: Venn diagram showing that miR-30c targets twinfilin 1 (TWF1) by three prediction tool intersections. B: Expression of TWF1 detected by quantitative real-time polymerase chain reaction after control or miR-30c mimics were transfected into pancreatic cancer cells. C: Expression of TWF1 detected by Western blot analysis after control or miR-30c mimics were transfected into pancreatic cancer cells. D: Predicted miR-30c target sequence in the TWF1 3’-UTR based on the TargetScan database. E: Relative luciferase activity of dual-luciferase reporter plasmids carrying wild-type or mutant TWF1 3’-UTR in pancreatic cancer cells cotransfected with control or miR-30c mimics. All experiments were performed at least three times. The data are presented as the mean ± standard deviation. P < 0.05 was considered statistically significant. ^aP < 0.05 vs mimics group; ^bP < 0.01 vs mimics group. Twinfilin 1: TWF1; 3’-UTRs: 3’-untranslated regions; WT: Wild-type; Mut: Mutant.