MiR-32-5p aggravates intestinal epithelial cell injury in pediatric enteritis induced by Helicobacter pylori

Figure 4 MiR-32-5p/SMAD family member 6 is involved in transforming growth factor-β-activated kinase 1-p38 pathway in intestinal epithelial cells. A: Detection of transforming growth factor-β-activated kinase 1 (TAK1)-p38 activation in Helicobacter pylori (H. pylori)-treated intestinal epithelial cells by Western blot; B: Inhibition of TAK1-p38 activation in H. pylori-treated intestinal epithelial cells with SMAD6 overexpression as revealed by Western blot; C: SMAD6 expression in intestinal epithelial cells treated with control serum and patient serum (PS); D: SMAD6 expression in PS-treated intestinal epithelial cells with miR-32-5p antagonist transfection; E: TAK1-p38 activation in PS-treated intestinal epithelial cells as revealed by Western blot; F: Inhibition of TAK1-p38 activation in PS-treated intestinal epithelial cells with miR-32-5p antagonist transfection as revealed by Western blot. CS: Control serum; PS: Patient serum; SMAD6: SMAD family member 6; TAK1: Transforming growth factor-β-activated kinase 1; H. pylori: Helicobacter pylori.