Nucleus tractus solitarius mediates hyperalgesia induced by chronic pancreatitis in rats

Figure 5 Trinitrobenzene sulfonic acid treatment results in accumulation of postsynaptic N-methyl D-aspartate receptor subtype 2B and α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor subtype 1 in the caudal nucleus tractus solitarius. A: Representative electron microscopy images showing immune-gold particles labeled N-methyl-D-aspartate receptor subtype 2B (NR2B) distributed in an asymmetric synapse within the caudal nucleus tractus solitarius (NTS) of sham (left) and chronic pancreatitis (CP) (right) rats; B: Percentage of NR2B particles in 150 nm bins as a function of distance from the nearest edge of synapses; C: Averaged distances of NR2B from the edge of synapses of sham rats and CP rats; D: Representative electron microscopy images showing immune-gold particles labeled GluR1 distributed in an asymmetric synapse within caudal NTS of sham (left) and CP (right) rats; E: Percentage of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor subtype 1 (GluR1) particles in 150 nm bins as a function of distance from the nearest edge of synapses; F: Averaged distances of GluR1 from the edge of synapses of sham rats and CP rats. Bar = 250 nm in A and D. ^aP < 0.05, ^cP < 0.001, TNBS vs sham. Ax: Axon; Den: Dendrite; GluR1: α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor subtype 1; NR2B: N-methyl-D-aspartate receptor subtype 2B; TNBS: Trinitrobenzene sulfonic acid.