Nucleus tractus solitarius mediates hyperalgesia induced by chronic pancreatitis in rats

Figure 4 Trinitrobenzene sulfonic acid treatment upregulates the expression of N-methyl D-aspartate receptor subtype 2B and α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor subtype 1 within the caudal nucleus tractus solitarius. A: Representative Western blots for NR2B and GluR1 within the caudal nucleus tractus solitarius (NTS) on postoperative days (POD) 7, 14, and 28; B: The amount of NR2B within the caudal NTS was significantly increased on POD 7, 14, and 28 after trinitrobenzene sulfonic acid (TNBS) treatment; C: The amount of GluR1 within the caudal NTS was significantly increased on POD 7, 14, and 28 after TNBS treatment; D: Representative Western blots for pNR2B and pGluR within the caudal NTS after TNBS treatment; E: The amount of pNR2B within the caudal NTS was significantly increased after TNBS treatment; F: The amount of pGluR1 within the caudal NTS was significantly increased after TNBS treatment. n = 3 rats per each group, one-way ANOVA, ^aP < 0.05, ^bP < 0.01, ^cP < 0.001, TNBS vs sham. GluR1: α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor subtype 1; NR2B: N-methyl-D-aspartate receptor subtype 2B; TNBS: Trinitrobenzene sulfonic acid.