Insulin-like growth factor 2 mRNA-binding protein 1 promotes cell proliferation via activation of AKT and is directly targeted by microRNA-494 in pancreatic cancer

Figure 5 Insulin-like growth factor 2 mRNA-binding protein 1 is a target of miR-494. A: Intersections among miRNA target prediction algorithms; B: Pancreatic cancer tissues displayed significantly lower miR-494 and miR-326 levels than the matched adjacent nontumor tissues; C: A schematic diagram of miR-494 binding sites in the 3’ untranslated region of insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1) from TargetScan; D: MiR-494 mimics or inhibitors were transfected into Panc-1 and Panc 0327 cells, respectively, and miR-494 expression was determined by quantitative reverse transcription polymerase chain reaction (RT-qPCR). U6 served as the internal reference; E-F: IGF2BP1 expression was determined by RT-qPCR after transfection in Panc-1 and Panc 0327 cells. GAPDH served as the internal reference; G-H: The relative luciferase activity was measured in Panc-1 and Panc 0327 cells after cotransfection. All data are expressed as the mean ± standard deviation from three independent experiments at least and P < 0.05 was considered statistically significant. ^aP < 0.05 vs normal group, ^bP < 0.001 vs normal group; ^cP < 0.001 vs control group of Panc-1 cells; ^dP < 0.001 vs control group of Panc 0327 cells; ^eP < 0.01 vs control group of Panc-1 cells; ^fP < 0.01 vs control group of Panc 0327 cells; ^gP < 0.01 vs control group; ^hP < 0.05 vs control group; ⁱP < 0.05 vs control group; ^jP < 0.01 vs control group; ^kP < 0.05 vs WT group; ^lP < 0.01 vs WT group; ^mP < 0.01 vs WT group; ⁿP < 0.01 vs WT group. IGF2BP1: Insulin-like growth factor 2 mRNA-binding protein 1; 3’-UTR: 3’-untranslated region; Wt: Wild-type; Mut: Mutated.