Insulin-like growth factor 2 mRNA-binding protein 1 promotes cell proliferation via activation of AKT and is directly targeted by microRNA-494 in pancreatic cancer

Figure 3 Knockdown of insulin-like growth factor 2 mRNA-binding protein 1 inhibits pancreatic cancer cell proliferation by inducing apoptosis and G1-phase arrest. A-C: Enrichment plots of gene expression signatures in the GSE62165 dataset according to insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1) mRNA expression levels; D: Knockdown of IGF2BP1 induced apoptosis in Panc-1 and Panc 0327 cells; E: Overexpression of IGF2BP1 reduced apoptosis in Mia PaCa-2 cells; F-H: Knockdown of IGF2BP1 induced G0/G1 phase arrest in Panc-1 and Panc 0327 cells and overexpression of IGF2BP1 promoted cell cycle progression in Mia PaCa-2 cells. All data are expressed as the mean ± standard deviation from three independent experiments at least and P < 0.05 was considered statistically significant. ^aP < 0.01 vs control group of Panc-1 cells; ^bP < 0.01 vs control group of Panc 0327 cells; ^cP < 0.001 vs control group of Mia PaCa-2 cells; ^dP < 0.05 vs control group of Panc-1 cells; ^eP < 0.05 vs control group of Panc 0327 cells; ^fP < 0.05 vs control group of Mia PaCa-2 cells. IGF2BP1: Insulin-like growth factor 2 mRNA-binding protein 1.