Role of tristetraprolin phosphorylation in paediatric patients with inflammatory bowel disease

Figure 2 Tristetraprolin, 14-3-3 protein and tumor necrosis factor-α expression in macrophages of paediatric inflammatory bowel disease patients and healthy donors. A: Tristetraprolin (TTP) immunoprecipitated, 14-3-3 co-immunoprecipitated and tumor necrosis factor-α expressions were normalized with actin (ACT) used as loading control. Mann–Whitney test TTP ^aP < 0.05, 14-3-3 ^aP < 0.05; B: Representative image of immunoblot experiments. IBD: Inflammatory bowel disease; HD: Healthy donors; TTP: Tristetraprolin; IP: Immunoprecipitated; Co-IP; Co-immunoprecipitated; TNF-α: Tumor necrosis factor-α; ACT: Actin.